截头多肽矩阵
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2019-11-16 17:53:51
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<p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255);"><span style="box-sizing: border-box; font-weight: 700;">截头多肽矩阵(Truncation Peptide Library)</span>:&nbsp;用于确定表位的最小范围。截头多肽矩阵的建立方法一般是系统性地去除原多肽序列两侧氨基酸而得到的。截头多肽矩阵用于鉴定与活性有关的最少氨基酸序列。</p><p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255);"><br/></p><p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255); text-align: center;"><img src="/upfiles/2019/1573898041119869.png" title="1477901299902496.png" alt="图片2.png" style="box-sizing: border-box; border: 0px; vertical-align: middle;"/></p><p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255);">&nbsp; &nbsp;这种肽库尺度的设计主要由N端截头数目(N-terminal Truncation)和C端截头数目(N-terminal Truncation)决定。</p><p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255);">&nbsp; &nbsp;你只需要输入蛋白序列及N端截头数目(N-terminal Truncation)和C端截头数目(N-terminal Truncation)数据,我们的软件可直接输出多肽序列。</p><p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255);">&nbsp; &nbsp;需要设计截头多肽矩阵点击此处。</p><p><br/></p>

上一篇:<p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255);"><span style="box-sizing: border-box; font-weight: 700;">Ala筛选矩阵(Alanine Peptide Scanning Library)</span>:&nbsp;用于鉴定多肽各位点对生物活性的影响。Ala被系统性地、依次地替代多肽序列中的每一个氨基酸。通过Ala筛选肽库以辨出某一特定氨基酸对整个蛋白结构、功能和其他生物活性的影响。</p><p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255); text-align: center;"><img src="/upfiles/2019/1573898014244162.png" title="1477901413826198.png" alt="图片3.png" style="box-sizing: border-box; border: 0px; vertical-align: middle;"/></p><p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255);">&nbsp; &nbsp;这种肽库尺度的设计主要由肽长决定。</p><p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255);">&nbsp; &nbsp;你只需要输入多肽序列,我们的软件可直接输出Ala筛选矩阵。</p><p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255);">&nbsp; &nbsp;需要设计Ala筛选矩阵点击此处。</p><p><br/></p>

下一篇:<p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255);">&nbsp;主要用于全蛋白扫描。首尾相搭的多肽矩阵是用于筛选线性或连续性表位的一种常用手段。这种肽库的设计主要由肽长(peptide length)和步移(offset number) 两个关键参数决定。蛋白从N端开始,逐步向C端截取设计后,最后很有可能会剩下一条孤肽(Orphan peptide),我们建议一般是将N端转移一个或者几个氨基酸过来,以保持一致长度。</p><p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255); text-align: center;"><img src="/upfiles/2019/1573898063420377.png" title="1477900883589663.png" alt="图片1.png" style="box-sizing: border-box; border: 0px; vertical-align: middle;"/></p><p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255);"><br style="box-sizing: border-box;"/></p><p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255);"><span style="box-sizing: border-box; font-family: 宋体;"><span style="box-sizing: border-box;">&nbsp; &nbsp;这种肽库尺度的设计主要由两个关键参数决定</span>:<span style="box-sizing: border-box;">肽长</span><span style="box-sizing: border-box; font-family: Calibri;">(peptide length)</span><span style="box-sizing: border-box;">和步移</span><span style="box-sizing: border-box; font-family: Calibri;">(offset number)</span><span style="box-sizing: border-box;">。你只需要输入蛋白序列及肽长</span><span style="box-sizing: border-box; font-family: Calibri;">(peptide length)</span><span style="box-sizing: border-box;">和步移</span><span style="box-sizing: border-box; font-family: Calibri;">(offset number)</span><span style="box-sizing: border-box;">数据,我们的软件可直接输出多肽序列。</span></span></p><p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 10px; color: rgb(51, 51, 51); font-family: arial, &quot;Microsoft YaHei&quot;, 微软雅黑, MicrosoftJhengHei, 华文细黑, STHeiti, MingLiu, 宋体, serif; font-size: 14px; background-color: rgb(255, 255, 255);"><span style="box-sizing: border-box; font-family: 宋体;"><span style="box-sizing: border-box;">&nbsp; &nbsp;</span></span></p>

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